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MP 5 Medium (Mineral Pectin 5 Medium)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MP 5 Medium

(Mineral Pectin 5 Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar solution .............................................. 500.0mL

Basal medium ............................................. 250.0mL

Mineral solution.......................................... 250.0mL

pH 5.0–6.0 at 25°C

Agar Solution:

Composition per 500.0mL:

Agar ..................................................................15.0g

Preparation of Agar Solution: Add agar to dis

tilled/deionized water and bring volume to 500.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Basal Medium:

Composition per 250.0mL:

Na2HPO4.............................................................6.0g

Pectin, citrus or apple .........................................5.0g

KH2PO4...............................................................4.0g

NH4SO4...............................................................2.0g

Yeast extract........................................................1.0g

Preparation of Basal Medium: Add compo

nents to distilled/deionized water and bring volume

to 250.0mL. Mix thoroughly. Gently heat and bring

to boiling.

Mineral Solution:

Composition per 250.0mL:

FeSO4 (0.1% solution).................................... 1.0mL

MgSO4·7H2O (20% solution) ......................... 1.0mL

CaCl2·2H2O (0.1% solution)........................... 1.0mL

H3BO3 (0.001% solution) ............................... 1.0mL

MnSO4·H2O (0.001% solution) ...................... 1.0mL

ZnSO4·7H2O (0.007% solution....................... 1.0mL

CuSO4·5H2O (0.005% solution) ..................... 1.0mL

MoO3 (0.001% solution)................................. 1.0mL

Preparation of Mineral Solution: Add compo

nents to distilled/deionized water and bring volume

to 250.0mL. Mix thoroughly.

Preparation of Medium: Combine 250.0mL of

basal medium and 250.0mL of mineral solution. Mix

thoroughly. Adjust pH to 5.0–6.0 with 1N HCl. Auto

clave the basal medium-mineral solution and agar solu

tion separately for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically combine the two sterile

solutions. Mix thoroughly. Pour immediately into ster

ile Petri dishes to prevent hydrolysis of the agar.

Use: For the cultivation of microorganisms that pro

duce polygalactanase.

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MP 5 Medium (Mineral Pectin 5 Medium)