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MP 7 Medium (Mineral Pectin 7 Medium)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MP 7 Medium

(Mineral Pectin 7 Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Basal medium ............................................. 500.0mL

Mineral solution.......................................... 500.0mL

pH 7.2 ± 0.2 at 25°C

Basal Medium:

Composition per 500.0mL:

Agar ..................................................................15.0g

Na2HPO4 .............................................................6.0g

Pectin, citrus or apple..........................................5.0g

KH2PO4...............................................................4.0g

NH4SO4...............................................................2.0g

Yeast extract........................................................1.0g

Preparation of Basal Medium: Add compo

nents to distilled/deionized water and bring volume

to 500.0mL. Mix thoroughly. Gently heat and bring

to boiling.

Mineral Solution:

Composition per 500.0mL:

FeSO4 (0.1% solution) .................................... 1.0mL

MgSO4·7H2O (20% solution) ......................... 1.0mL

CaCl2·2H2O (0.1% solution)........................... 1.0mL

H3BO3 (0.001% solution) ............................... 1.0mL

MnSO4·H2O (0.001% solution) ...................... 1.0mL

ZnSO4·7H2O (0.007% solution....................... 1.0mL

CuSO4·5H2O (0.005% solution ...................... 1.0mL

MoO3 (0.001% solution)................................. 1.0mL

Preparation of Mineral Solution: Add compo

nents to distilled/deionized water and bring volume

to 500.0mL. Mix thoroughly.

Preparation of Medium: Combine 500.0mL of

basal medium and 500.0mL of mineral solution. Mix

thoroughly. Adjust pH to 7.2. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°C. Pour into

sterile Petri dishes.

Use: For the cultivation of microorganisms that pro

duce pectate lyase.