培养基配方

您现在的位置：网站首页 >> 培养基配方

MRS Broth with CaCO3 (LMG Medium 166)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Broth with CaCO3

(LMG Medium 166)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

CaCO3 ...............................................................30.0g

Glucose .............................................................20.0g

Peptone .............................................................10.0g

Beef extract.........................................................5.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................2.0g

Diammonium hydrogen citrate...........................2.0g

MgSO4·7H2O......................................................0.2g

MnSO4·4H2O.................................................38.0mg

Sorbitan monooleate ....................................... 1.0mL

pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation of Sporolactobacillus inulinus.