培养基配方

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MRS Broth with Cysteine

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................18.5g

Pancreatic digest of gelatin...............................10.0g

Beef extract.........................................................8.0g

Yeast extract........................................................4.0g

Sodium acetate....................................................3.0g

K2HPO4...............................................................2.0g

Ammonium citrate ..............................................2.0g

Polysorbate 80.....................................................1.0g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O ....................................................0.05g

L-Cysteine solution ......................................... 0.5mL

pH 6.2 ± 0.2 at 25°C

L-Cysteine Solution:

Composition per 10.0mL:

L-Cysteine ...........................................................0.1g

Preparation of L-Cysteine Solution: Add 0.1g

of L-cysteine to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept L-cysteine solution, to distilled/deionized water

and bring volume to 999.5mL. Mix thoroughly. Ad

just pH to 6.2. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 0.5mL of sterile L

cysteine solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Leuconos

toc mesenteroides.