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MRS Broth with Ethanol (LMG Medium 240)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Broth with Ethanol

(LMG Medium 240)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone..............................................................10.0g

Beef extract.........................................................5.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Diammonium hydrogen citrate ...........................2.0g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O .................................................38.0mg

Ethanol........................................................ 100.0mL

Tween 80......................................................... 1.0mL

pH 5.0± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept ethanol, to distilled/deionized water and bring

volume to 900.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add 100.0mL of sterile ethanol. Mix

thoroughly. Aseptically distribute into sterile tubes or

sterile flasks.

Use: For the cultivation of Lactobacillus acetotolerans.