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MRS Broth with Ethanol

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone..............................................................10.0g

Beef extract.........................................................8.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................4.0g

K2HPO4...............................................................2.0g

Triammonium citrate ..........................................2.0g

MgSO4·7H2O......................................................0.2g

MnSO4·4H2O....................................................0.05g

Sorbitan monooleate ....................................... 1.0mL

Ethanol (95% solution),

filter sterilized ...................................... 100.0mL

pH 5.0 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept ethanol, to distilled/deionized water and bring

volume to 900.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Aseptically add

100.0mL of sterile ethanol. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

Use: For the cultivation of Lactobacillus acetotolerans.