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Potato Dextrose Agar and Yeast Medium (PDA and Yeast Medium) (ATCC Medium 104)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar and Yeast Medium

(PDA and Yeast Medium)

(ATCC Medium 104)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

KH2PO4...............................................................2.0g

Yeast extract........................................................1.5g

MgSO4·7H2O......................................................0.5g

Potato infusion............................................ 500.0mL

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potatoes, Infusion From: Peel

and dice potatoes. Add 500.0mL of distilled/deion

ized water. Gently heat and bring to boiling. Contin

ue boiling for 30 min. Filter through cheesecloth.

Reserve filtrate.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and maintenance of

Pseudomonas fluorescens, Streptoverticillium bal

daccii, and Xanthomonas campestris.