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Potato Dextrose Agar with Antibiotics

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potato, infusion from ..........................................4.0g

Antibiotic solution ........................................ 20.0mL

Antibiotic Solution:

Composition per 100.0mL:

Chlortetracycline·HCl .........................................0.5g

Chloramphenicol.................................................0.5g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept antibiotic solution, to distilled/deionized water

and bring volume to 980.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add 20.0mL of sterile antibiotic solution. Mix thor

oughly. Pour into sterile Petri dishes.

Use: For the cultivation of fungi from foods.