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Potato Dextrose Agar with Gentamycin (ATCC Medium 2286)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar with Gentamycin

(ATCC Medium 2286)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potatoes, infusion from............................... 500.0mL

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potato Infusion: Peel and dice

potatoes. Add 500.0mL of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

Gentamycin Solution:

Composition per 10.0mL:

Gentamycin.....................................................100mg

Preparation of Gentamycin Solution: Add

gentamycin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept gentamycin to distilled/deionized water and

bring volume to 990.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

10.0mL of sterile gentamycin solution. Mix thor

oughly. Pour into sterile Petri dishes or sterile tubes.

Use: For the cultivation of yeasts and molds from

dairy products and other foods.