培养基配方

您现在的位置：网站首页 >> 培养基配方

Potato Dextrose L-Isoleucine Agar (ATCC Medium 2205)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose L-Isoleucine Agar

(ATCC Medium 2205)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potatoes, infusion from............................... 500.0mL

L-Isoleucine solution..................................... 50.0mL

pH 5.6 ± 0.2 at 25°C

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potato Infusion: Peel and dice

potatoes. Add 500.0mL of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

L-Isoleucine Solution:

Composition per 50.0mL:

L-Isoleucine.......................................................0.13g

Preparation of L-Isoleucine Solution: Add L

isoleucine to distilled/deionized water and bring vol

ume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept L-isoleucine solution to distilled/deionized wa

ter and bring volume to 950.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 50.0mL of sterile L-isoleucine solu

tion. Mix thoroughly. Pour into sterile Petri dishes or

distribute to sterile tubes.

Use: For the cultivation of yeasts and molds.