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Potato Dextrose Salt Agar (BAM M127)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Salt Agar

(BAM M127)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................75.0g

Agar ..................................................................20.0g

Glucose .............................................................20.0g

Potato infusion................................................... 1.0L

pH 5.6 ± 0.2 at 25°C

Potato Infusion:

Composition per 1.0L:

Potatoes, unpeeled and sliced .........................200.0g

Preparation of Potato Infusion: Add unpeeled

potato slices to 1.0L of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

Bring volume to 1.0L with distilled/deionized water.

Preparation of Medium: Add agar and glucose

to 1.0L potato infusion. Mix thoroughly. Gently heat

and bring to boiling. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of holo

philic yeasts and halophilic filamentous fungi

(molds) from foods.