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Potato Dextrose Yeast Agar (PDY Agar)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Yeast Agar

(PDY Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Yeast extract........................................................5.0g

Potato infusion............................................ 500.0mL

pH 5.6 ± 0.2 at 25°C

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potato Infusion: Peel and dice

potatoes. Add 500.0mL of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and maintenance of Bacillus

species and fungi; also used to induce sporulation in

many fungi.