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Preenrichment Medium (PEM)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Preenrichment Medium

(PEM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract......................................................20.0g

Special peptone, Oxoid.....................................10.0g

Na2HPO4 .............................................................7.1g

NaCl....................................................................1.0g

KCl......................................................................1.0g

MgSO4·7H2O solution .................................. 10.0mL

CaC2·2H2O solution...................................... 10.0mL

pH 8.3 ± 0.2 at 25°C

MgSO4·7H2O Solution:

Composition per 10.0mL:

MgSO4·7H2O ...................................................0.01g

Preparation of MgSO4·7H2O Solution: Add

MgSO4·7H2O to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

CaCl2·2H2O Solution:

CaC2·2H2O........................................................0.01g

Preparation of CaCl2·2H2O Solution: Add the

CaCl2·2H2O to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

MgSO4·7H2O solution and CaCl2·2H2O solution, to

distilled/deionized water and bring volume to 980.0mL.

Mix thoroughly. Adjust pH to 8.3. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile

MgSO4·7H2O solution and CaCl2·2H2O solution. Mix

thoroughly. Aseptically distribute into sterile tubes.

Use: For the isolation and enrichment of Yersinia en

terocolitica from foods.