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Preston Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef extract.......................................................10.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Horse blood, lysed ........................................ 50.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.5 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Rifampicin ........................................................0.01g

Trimethoprim lactate.........................................0.01g

Polymyxin B ...................................................5000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept horse blood and antibiotic solution, to distilled/

deionized water and bring volume to 940.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile horse blood and

antibiotic solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the isolation and enrichment of Campylo

bacter species from foods.