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Propionibacterium Medium

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract......................................................10.0g

Na2HPO4·2H2O...................................................3.0g

KH2PO4...............................................................1.0g

Lactate solution............................................. 40.0mL

pH 7.0 ± 0.2 at 25°C

Lactate Solution:

Composition per 100.0mL:

Sodium lactate ..................................................70.0g

Preparation of Lactate Solution: Add 70.0g of

sodium lactate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept lactate solution, to distilled/deionized water and

bring volume to 960.0mL. Mix thoroughly. Add

40.0mL of lactate solution. Mix thoroughly. Adjust

pH to 7.0. Distribute into screw-capped tubes. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Propionibacterium acid

ipropionici, Propionibacterium freudenreichii, Pro

pionibacterium jensenii, and Propionibacterium

thoenii.