培养基配方

您现在的位置：网站首页 >> 培养基配方

Proskauer-Beck Medium for Mycobacterium

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Proskauer-Beck Medium

for Mycobacterium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Asparagine ..........................................................5.0g

KH2PO4...............................................................5.0g

Magnesium citrate ..............................................2.5g

MgSO4·7H2O......................................................0.6g

Glycerol ........................................................ 20.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, one

at a time, to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Make sure one salt is

totally dissolved before the next one is added. Adjust

pH to 7.8 with 40% NaOH. Autoclave for 15 min at

15 psi pressure–121°C. The pH of the medium after

autoclaving should be 7.4. Filter through Whatman

#1 filter paper to remove any precipitate. Distribute

into tubes or flasks. Autoclave again for 15 min at 15

psi pressure–121°C.

Use: For the cultivation and maintenance of Myco

bacterium tuberculosis.