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Pseudomonas Basal Mineral Medium

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

K2HPO4.............................................................12.5g

KH2PO4...............................................................3.8g

(NH4)2SO4 ..........................................................1.0g

MgSO4·7H2O......................................................0.1g

Carbon source (0.8M solution) ................... 100.0mL

Trace element solution.................................... 5.0mL

pH 7.2 ± 0.2 at 25°C

Trace Element Solution:

Composition per liter:

H3BO3 .............................................................0.232g

ZnSO4·7H2O ...................................................0.174g

FeSO4(NH4)2SO4·6H2O..................................0.116g

CoSO4·7H2O...................................................0.096g

(NH4)6Mo7O24·4H2O ......................................0.022g

CuSO4·5H2O....................................................8.0mg

MnSO4·4H2O...................................................8.0mg

Preparation of Trace Element Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly.

Carbon Source:

Composition per 100.0mL:

Glucose .............................................................14.4g

Preparation of Carbon Source: Add glucose to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize. Other car

bon sources may replace glucose. Prepare 0.8M car

bon source solution.

Preparation of Medium: Add components, ex

cept carbon source, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 100.0mL of sterile carbon source. Mix thorough

ly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and differentiation of

Pseudomonas species based on their ability to grow

on different carbon sources.