培养基配方

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Pseudomonas CFC Agar

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................16.0g

Agar ..................................................................11.0g

Pancreatic digest of casein................................10.0g

K2SO4 ...............................................................10.0g

MgCl2·6H2O .......................................................1.4g

CFC selective supplement ............................ 10.0mL

Glycerol ........................................................ 10.0mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

CFC Selective Supplement:

Composition per 10.0mL:

Cephaloridine....................................................0.05g

Fucidin ..............................................................0.01g

Cetrimide ..........................................................0.01g

Preparation of CFC Selective Supplement:

Add components to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept CFC selective supplement, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile CFC selective supplement.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the selective isolation and cultivation of

Pseudomonas species.