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Pseudomonas CN Agar

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas CN Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................16.0g

Agar ..................................................................11.0g

Pancreatic digest of casein................................10.0g

K2SO4................................................................10.0g

MgCl2·6H2O .......................................................1.4g

CN selective supplement .............................. 10.0mL

Glycerol ........................................................ 10.0mL

CN Selective Supplement:

Cetrimide ............................................................0.1g

Sodium nalidixate ............................................7.5mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components, ex

cept CN selective supplement, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile CN selective supplement. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the selective isolation and cultivation of

Pseudomonas species.