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PYG Broth (Peptone Yeast Extract Glucose Broth)

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

PYG Broth

(Peptone Yeast Extract Glucose Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................20.0g

D-Glucose..........................................................10.0g

Yeast extract......................................................10.0g

L-Cysteine·HCl·H2O ...........................................0.5g

VPI salt solution............................................ 40.0mL

Resazurin solution........................................... 4.0mL

pH 7.2 ± 0.2 at 25°C

Resazurin Solution:

Composition per 44.0mL:

Resazurin ........................................................0.044g

Preparation of Resazurin Solution: Add resa

zurin to distilled/deionized water and bring volume

to 44.0mL. Mix thoroughly.

VPI Salt Solution:

Composition per 40.0mL:

CaCl2...................................................................0.2g

MgSO4 ................................................................0.2g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

Preparation of VPI Salt Solution: Add CaCl2

and MgSO4 to 300.0mL of distilled/deionized water.

Mix thoroughly until dissolved. Bring volume to

800.0mL with distilled/deionized water. Add remain

ing components while stirring. Bring volume to 1.0L.

Mix thoroughly. Store at 4°C.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into screw-capped tubes

in 7.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C under 100% N2.

Use: For the cultivation of a wide variety of anaero

bic bacteria.