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Pyrazinamidase Agar (BAM M131)

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrazinamidase Agar

(BAM M131)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..............................11.25g

Agar ................................................................11.25g

Papaic digest of soybean meal..........................3.75g

NaCl..................................................................3.75g

Yeast extract........................................................3.0g

Pyrazine-carboxamide ........................................1.0g

Tris maleate, 0.2M, pH6.0 ................................. 1.0L

pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

0.2M tris maleate and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or

leave in tubes. For slants allow tubes to cool in an in

clined position.

Use: For the cultivation of Yersinia spp.