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Pyruvate Utilization Medium

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

K2HPO4...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Bromthymol Blue ...............................................0.1g

pH 7.1–7.4 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Adjust pH to 7.1–7.4. Distribute into tubes in 5.0mL

volumes. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of bacteria that can metabo

lize pyruvate. Bacteria that can utilize pyruvate turn

the medium yellow.