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Rambach Agar

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Rambach Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Polypropylene glycol ........................................10.5g

Peptone................................................................8.0g

NaCl....................................................................5.0g

Na-deoxycholate .................................................1.0g

Chromogenic mix ...............................................1.5g

pH 7.4 ± 0.2 at 25°C

Source: Rambach Agar is available from CHROM

agar Microbiology.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat in a boiling water bath

or steam bath. Shake periodically during heating to

dissolve components. Heating for 40 min with shak

ing every 5 min should ensure complete dissolution.

Do not overheat. Cool to 45-50°C. Pour into sterile

Petri dishes.

Use: For the differentiation of non-typhi Salmonella

spp. in foodstuffs. Salmonellae form acid with propy

lene glycol and non-typhi Salmonella spp. form red col

onies. Coliform bacteria grow as blue-green or blue-vi

olet colonies. Other Enterobacteriaceae and Gram neg

ative bacteria, such as Proteus spp., Pseudomonas spp.,

Shigella spp., Salmonella typhi, and Salomonella

paratyphi A grow as colorless or yellow colonies.