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Rambach® Agar

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Rambach® Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaC....................................................................l5.0g

Agar ..................................................................15.0g

Proplylene glycol..............................................10.5g

Peptone ...............................................................8.0g

Chromogenic mix ...............................................1.5g

Sodium deoxycholate..........................................1.0g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Heat in a boiling water-batch or in a

current of steam, while shaking from time to time.

The medium is totally suspended, if no visual parti

cles stick to the glass-wall. The medium should not

be heat-treated further. Complete dissolution with

shaking in 5-min sequences is approximately 35-40

min. Do not autoclave. Do not overheat. Cool as fast

as possible to 45°–50°C while gently shaking from

time to time. Pour into sterile Petri dishes. To prevent

any precipitate or clotting of the chromogenic-mix in

the plates, place Petri dishes during pouring proce

dure on a cool (max. 25°C) surface. The plates are

opaque and pink.

Use: For the detection of enteric bacteria, including

coliforms and Salmonella spp. Sodium deoxycholate

inhibits the accompanying Gram-positive flora. This

medium enables Salmonella spp. to be differentiated

unambiguously from other bacteria. Salmonella spp.

form a characteristic red color. In order to differenti

ate coliforms from Salmonellae, the medium con

tains a chromogene indicating the presence of β-ga

lactosidase splitting, a characteristic for coliforms.

Coliform microorganisms grow as blue-green or

blue-violet colonies. Other Enterobacteriaceae and

Gram-negative bacteria, such as Proteus, Pseudomo

nas, Shigella, S. typhi, and S. parathyphi A grow as

colorless-yellow colonies.