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Rappaport Broth, Modified (Rap Broth, Modified)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Rappaport Broth, Modified

(Rap Broth, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 250.2mL:

Solution A................................................... 155.0mL

Solution C ..................................................... 53.0mL

Solution B ..................................................... 40.0mL

Solution D....................................................... 1.6mL

Solution E ....................................................... 0.6mL

Solution A:

Composition per liter:

Pancreatic digest of casein................................10.0g

Preparation of Solution A: Add pancreatic di

gest of casein to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly.

Solution B:

Composition per liter:

Na2HPO4.............................................................9.5g

Preparation of Solution B: Add Na2HPO4 to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly.

Solution C:

Composition per 100.0mL:

MgCl2·6H2O .....................................................40.0g

Preparation of Solution C: Add MgCl2·6H2O to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C.

Solution D:

Composition per 100.0mL:

Malachite Green..................................................0.2g

Preparation of Solution D: Add Malachite

Green to sterile distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Do not steril

ize.

Solution E:

Composition per 10.0mL:

Carbenicillin .....................................................0.01g

Preparation of Solution E: Add carbenicillin to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Combine 155.0mL of

solution A and 40.0mL of solution B. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 53.0mL of sterile

solution C, 1.6mL of solution D, and 0.6mL of sterile

solution E. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the isolation and cultivation of Yersinia en

terocolitica from foods.

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Rappaport Broth, Modified (Rap Broth, Modified)