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Rappaport-Vassiliadis Enrichment Broth (RV Enrichment Broth) (BAM M132)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Rappaport-Vassiliadis

Enrichment Broth

(RV Enrichment Broth)

(BAM M132)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1110.0mL:

NaCl....................................................................8.0g

Papaic digest of soybean meal............................5.0g

KH2PO4...............................................................1.6g

Magnesium chloride solution...................... 100.0mL

Malachite Green solution.............................. 10.0mL

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Magnesium Chloride Solution:

Composition per 100.0mL:

MgCl2·6H2O .....................................................40.0g

Preparation of Magnesium Chloride Solu

tion: Add MgCl2·6H2O to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Malachite Green Solution:

Composition per 10.0mL:

Malachite Green oxalate ...................................0.04g

Preparation of Malachite Green Solution:

Add Malachite Green to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes in 10.0mL vol

umes. Autoclave for 15 min at 10 psi pressure–

115°C.

Use: For the isolation and cultivation of Salmonella

species from foods.