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Rimler-Shotts Medium (RS Medium)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Rimler-Shotts Medium

(RS Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

Na2S2O3·5H2O ....................................................6.8g

L-Ornithine·HCl ..................................................6.5g

NaCl....................................................................5.0g

L-Lysine·HCl .......................................................5.0g

Maltose................................................................3.5g

Yeast extract........................................................3.0g

Sodium deoxycholate..........................................1.0g

Ferric ammonium citrate.....................................0.8g

L-Cysteine·HCl....................................................0.3g

Bromthymol Blue .............................................0.03g

Novobiocin solution...................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin.......................................................5.0mg

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile components. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation, cultivation, and pre

sumptive identification of Aeromonas hydrophila

and other Gram-negative bacteria based on their abil

ity to decarboxylate lysine and ornithine, ferment

maltose, and produce H2S. Maltose-fermenting bac

teria appear as yellow colonies. Bacteria that produce

lysine or ornithine decarboxylase turn the medium

greenish-yellow to yellow. Bacteria that produce H2S

appear as colonies with black centers.