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Rippey-Cabelli Agar (RC Agar)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Rippey-Cabelli Agar

(RC Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Meat peptone ......................................................5.0g

Trehalose.............................................................5.0g

NaCl....................................................................3.0g

KCl......................................................................2.0g

Yeast extract........................................................2.0g

Bromthymol Blue .............................................0.44g

MgSO4·7H2O......................................................0.2g

FeCl3·6H2O.........................................................0.1g

Sodium deoxycholate..........................................0.1g

Ampicillin solution ....................................... 10.0mL

Ethanol.......................................................... 10.0mL

pH 8.0 ± 0.2 at 25°C

Ampicillin Solution:

Composition per 10.0mL:

Ampicillin.........................................................0.02g

Preparation of Ampicillin Solution: Add

ampicillin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept sodium deoxycholate, ampicillin solution, and

ethanol—to distilled/deionized water and bring vol

ume to 990.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add so

dium deoxycholate, 10.0mL of sterile ampicillin

solution, and 10.0mL of ethanol. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the isolation, cultivation, and differentia

tion of Aeromonas species and Plesiomonas species

from water samples using the membrane filter meth

od. This medium differentiates bacteria on the basis

of trehalose fermentation. Bacteria that ferment treh

alose turn the medium yellow.