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Rogosa Agar

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Rogosa Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate..................................................25.0g

Agar ..................................................................20.0g

Glucose .............................................................20.0g

Pancreatic digest of casein................................10.0g

KH2PO4...............................................................6.0g

Yeast extract........................................................5.0g

Ammonium citrate..............................................2.0g

Sorbitan monooleate ...........................................1.0g

MgSO4·7H2O..................................................0.575g

MnSO4·H2O......................................................0.12g

FeSO4·7H2O.....................................................0.4mg

Acetic acid, glacial........................................ 1.32mL

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components, ex

cept acetic acid, to distilled/deionized water and

bring volume to 998.7mL. Mix thoroughly. Gently

heat and bring to boiling. Add glacial acetic acid.

Mix thoroughly. Gently heat while stirring and bring

to 90°–100°C for 2–3 min. Do not autoclave. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration

of lactobacilli, especially from dairy products.