培养基配方

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Rogosa Broth, Modified

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1005.0mL:

Glucose .............................................................20.0g

Trypticase..........................................................10.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................3.0g

KH2PO4...............................................................3.0g

Tryptose ..............................................................3.0g

Ammonium citrate ..............................................2.0g

Sodium acetate....................................................1.0g

Tween 80.............................................................1.0g

L-Cysteine ...........................................................0.2g

Salt solution .................................................... 5.0mL

pH 6.8 ± 0.2 at 25°C

Salt Solution:

Composition per 100.0mL:

MgSO4·7H2O ..................................................11.50g

MnSO4 ..............................................................2.40g

FeSO4·7H2O......................................................1.68g

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, ex

cept salt solution, to distilled/deionized water and

bring volume to 995.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 5.0mL of sterile salt solution. Adjust pH to

6.8. Mix thoroughly. Aseptically distribute into ster

ile tubes or flasks.

Use: For the cultivation of Lactobacillus species.