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Saccharolytic Clostridia Medium
[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]
Saccharolytic Clostridia Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein................................10.0g
Yeast extract........................................................6.0g
Sodium thioglycolate ..........................................0.5g
Carbohydrate solution................................. 100.0mL
Potassium phosphate
(1M solution, pH 7.5) ............................. 30.0mL
MgSO4 (1M solution) ..................................... 1.0mL
Solution M ...................................................... 0.5mL
FeSO4 solution ................................................ 0.2mL
pH 7.0–7.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Glucose or sucrose............................................20.0g
Preparation of Carbohydrate Solution: Add
carbohydrate to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution M:
Composition per liter:
CaCl2.................................................................3.33g
MnCl2·4H2O .....................................................1.98g
Na2MoO4·2H2O ................................................1.21g
CoCl2·6H2O ......................................................1.19g
Preparation of Solution M: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly.
FeSO4·7H2O Solution:
Composition per 10.0mL:
FeSO4·7H2O......................................................55.6g
H2SO4 (0.1M solution).................................. 10.0mL
Preparation of FeSO4·7H2O Solution: Add the
FeSO4·7H2O to 10.0mL of H2SO4 solution. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept carbohydrate solution, to distilled/deionized wa
ter and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 20
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile carbohydrate solution. Mix
thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation of saccharolytic Clostridium
species.