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Saccharomyces Medium

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone .............................................................20.0g

Agar ..................................................................15.0g

Yeast extract......................................................10.0g

Yeast nitrogen base without amino acids............6.7g

(NH4)2SO4 ..........................................................5.0g

Proline..............................................................4.0mg

Preparation of Medium: Add glucose, yeast ni

trogen base without amino acids, and proline to dis

tilled/deionized water and bring volume to 200.0mL.

Mix thoroughly. Filter sterilize. Add peptone, yeast

extract, (NH4)2SO4, and agar to distilled/deionized

water and bring volume to 800.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally combine the two sterile solutions. Mix thor

oughly. Pour into sterile Petri dishes or aseptically

distribute into sterile tubes.

Use: For the cultivation and maintenance of Saccha

romyces cerevisiae.