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Salt Tolerance Medium

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from................................500.0g

NaCl..................................................................65.0g

Tryptose ............................................................10.0g

Glucose ...............................................................1.0g

Indicator solution............................................ 1.0mL

pH 7.4 ± 0.2 at 25°C

Indicator Solution:

Composition per 100.0mL:

Bromcresol Purple ..............................................1.6g

Ethanol (95% solution)............................... 100.0mL

Preparation of Indicator Solution: Add Brom

cresol Purple to ethanol. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of salt-tolerant Streptococ

cus species and other salt-tolerant Gram-positive

cocci. For the differentiation of Gram-positive cocci

based on salt tolerance.