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Salt Polymyxin Broth (SPB)

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Salt Polymyxin Broth

(SPB)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................3.0g

Polymyxin B ..............................................250,000U

pH 8.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 8.8. Distribute into

tubes or flasks. Autoclave for 10 min at 10 psi pres

sure–115°C.

Use: For the isolation and cultivation of Vibrio spe

cies from foods.