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培养基配方
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SBG Sulfa Enrichment
[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]
SBG Sulfa Enrichment
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
D-Mannitol ..........................................................5.0g
Peptone................................................................5.0g
Yeast extract........................................................5.0g
Na2SeO3·5H2O....................................................4.0g
K2HPO4.............................................................2.65g
KH2PO4.............................................................1.02g
Sodium taurocholate ...........................................1.0g
Sodium sulfapyridine..........................................0.5g
Brilliant Green .................................................5.0mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Continue boiling for 5–10 min. Do not autoclave.
Distribute into sterile tubes or flasks.
Use: For the selective isolation of Salmonella spe
cies, especially from eggs and egg products.