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SC Agar

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

SC Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

(NH4)2SO4...........................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O.........................................................0.1g

Inositol .............................................................2.0mg

KI .....................................................................1.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O ...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyroxidine·HCl ................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

CuSO4·5H2O ..................................................0.04mg

Folic acid.......................................................... 2.0μg

Biotin ............................................................... 2.0μg

Glucose solution ........................................... 50.0mL

Synthetic stock solution................................ 30.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................40.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Synthetic Stock Solution:

Composition per 300.0mL:

L-Isoleucine.......................................................5.25g

L-Arginine.........................................................3.48g

L-Aspartic acid..................................................2.66g

L-Leucine ..........................................................2.62g

L-Methionine.....................................................1.49g

L-Threonine.......................................................1.19g

L-Valine.............................................................1.17g

L-Serine.............................................................1.05g

L-Lysine ............................................................0.91g

L-Phenylalanine ................................................0.83g

L-Tryptophan ....................................................0.82g

L-Histidine ........................................................0.58g

m-Inositol..........................................................0.36g

Uracil ................................................................0.22g

L-Tyrosine .........................................................0.18g

Adenine...........................................................0.135g

Preparation of Synthetic Stock Solution: Add

components to distilled/deionized water and bring

volume to 300.0mL. Mix thoroughly. Filter sterilize.

Store in dark at 25°C.

Preparation of Medium: Add components, ex

cept synthetic stock solution and glucose solution, to

distilled/deionized water and bring volume to

920.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C. Aseptically add 30.0mL

of sterile synthetic stock solution and 50.0mL of ster

ile glucose solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Saccha

romyces cerevisiae.

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SC Agar