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Schleifer-Krämer Agar (SK Agar)

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Schleifer-Krämer Agar

(SK Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.0g

Glycerol ............................................................10.0g

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

Yeast extract........................................................3.0g

Potassium isothiocyanate..................................2.25g

LiCl .....................................................................2.0g

Na2HPO4·2H2O...................................................0.9g

NaH2PO4·H2O.....................................................0.6g

Glycine................................................................0.5g

NaN3 solution................................................ 10.0mL

pH 7.2 ± 0.2 at 25°C

NaN3 Solution:

Composition per 10.0mL:

NaN3 ...............................................................0.045g

Preparation of NaN3 Solution: Add NaN3 to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept NaN3 solution, to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Adjust

pH to 7.2. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile NaN3 solution.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the isolation and cultivation of Staphylo

coccus species.