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Seawater Agar (SWA)

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Agar

(SWA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone................................................................5.0g

Yeast extract........................................................5.0g

Beef extract.........................................................3.0g

Seawater, synthetic ............................................ 1.0L

pH 7.5 ± 0.2 at 25°C

Seawater, Synthetic:

Composition per liter:

NaCl..................................................................27.0g

MgSO4·7H2O ......................................................7.0g

Tris(hydroxymethyl)aminomethane buffer.........2.0g

KCl......................................................................0.6g

CaCl2...................................................................0.3g

Preparation of Seawater, Synthetic: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the isolation and cultivation of halophilic

microorganisms from foods, such as Pseudomonas

species and Vibrio species from fish.