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Selenite Cystine Broth (BAM M134)
[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]
Selenite Cystine Broth
(BAM M134)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Na2HPO4.............................................................5.5g
Polypeptone ........................................................5.0g
KH2PO4...............................................................4.5g
Lactose................................................................4.0g
Na2SeO3·5H2O....................................................4.0g
L-Cystine ...........................................................0.01g
pH 7.0 ± 0.2 at 25°C
Caution: Sodium selenite is toxic and a potential
teratogen and care must be taken to avoid inhalation
of the powdered dye, contact with the skin, or inges
tion, especially in pregnant laboratory workers.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat. Do not autoclave. Dis
tribute into sterile tubes in 10.0mL volumes. Sterilize
for 10 min at 0 psi pressure–100°C in flowing steam.
Use: For the isolation and cultivation of Salmonella
species from feces, dairy products, and other speci
mens.