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Selenite F Broth

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Selenite F Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4...............................................................7.0g

Pancreatic digest of casein..................................5.0g

Lactose................................................................4.0g

Na2SeO3·5H2O....................................................4.0g

Na2HPO4.............................................................3.0g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Sodium selenite is toxic and a potential

teratogen and care must be taken to avoid inhalation

of the powdered dye, contact with the skin, or inges

tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat. Do not autoclave. Dis

tribute into sterile tubes in 10.0mL volumes. Sterilize

for 30 min at 0 psi pressure–100°C.

Use: For the isolation and cultivation of Salmonella

species from feces, dairy products, and other speci

mens.