培养基配方

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Semisolid Medium for Motility

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Biosate ................................................................5.0g

Polypeptone ........................................................5.0g

NaCl....................................................................5.0g

Agar ....................................................................4.0g

Myosate...............................................................1.5g

Triphenyltetrazolium chloride solution .......... 2.5mL

pH 6.9–7.1at 25°C

Triphenyltetrazolium Chloride Solution:

Composition per 10.0mL:

Triphenyltetrazolium chloride ............................0.1g

Ethanol (95% solution)................................. 10.0mL

Preparation of Triphenyltetrazolium Chlo

ride Solution: Add triphenyltetrazolium chloride

to 10.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components, ex

cept triphenyltetrazolium chloride solution, to dis

tilled/deionized water and bring volume to 997.5mL.

Mix thoroughly. Gently heat and bring to boiling.

Add 2.5mL of triphenyltetrazolium chloride solu

tion. Mix thoroughly. Distribute into tubes in 10.0mL

volumes. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the differentiation of bacteria based on mo

tility.