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Serum Glucose Agar (Serum Dextrose Agar) (ATCC Medium 287)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Serum Glucose Agar

(Serum Dextrose Agar)

(ATCC Medium 287)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1060.0mL:

Agar ..................................................................15.0g

Peptone .............................................................10.0g

Beef extract.........................................................5.0g

NaCl....................................................................5.0g

Serum-glucose solution ................................ 60.0mL

pH 7.3 ± 0.2 at 25°C

Serum-Glucose Solution:

Composition per 60.0mL:

D-Glucose..........................................................10.0g

Serum (inactivated at 56°C, 30 min) ............ 50.0mL

Preparation of Serum-Glucose Solution: Add

glucose to 50.0mL of heat-inactivated serum. Horse

serum or ox serum may be used. Mix thoroughly. Fil

ter sterilize.

Preparation of Medium: Add components, ex

cept serum-glucose solution, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 10 psi pressure–115°C. Cool to 50°C. Asepti

cally add 60.0mL of sterile serum-glucose solution.

Mix thoroughly. Pour into sterile Petri dishes or dis-

tribute into sterile tubes. Allow tubes to cool in a

slanted position.

Use: For the cultivation and maintenance of Brucel

la species.