- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置: 网站首页 >> 培养基配方
Serum Glucose Agar, Farrell Modified
[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]
Serum Glucose Agar, Farrell Modified
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1086.9mL:
Agar ..................................................................15.0g
Peptone..............................................................10.0g
Beef extract.........................................................5.0g
NaCl....................................................................5.0g
Serum-glucose solution................................. 60.0mL
Bacitracin solution ........................................ 12.5mL
Cycloheximide solution ................................ 10.0mL
Nystatin solution............................................. 2.0mL
Polymyxin B solution ..................................... 1.0mL
Nalidixic acid solution.................................... 1.0mL
Vancomycin solution....................................... 0.4mL
pH 7.3 ± 0.2 at 25°C
Serum-Glucose Solution:
Composition per 60.0mL:
D-Glucose ..........................................................10.0g
Serum (inactivated at 56°C, 30 min) ............ 50.0mL
Preparation of Serum-Glucose Solution: Add
glucose to 50.0mL of heat-inactivated serum. Horse
serum or ox serum may be used. Mix thoroughly. Fil
ter sterilize.
Bacitracin Solution:
Composition per 12.5mL:
Bacitracin.....................................................25,000U
Preparation of Bacitracin Solution: Add Baci
tracin to distilled/deionized water and bring volume
to 12.5mL. Mix thoroughly. Filter sterilize.
Cycloheximide Solution:
Composition per 100.0mL:
Cycloheximide....................................................1.0g
Acetone ........................................................... 5.0mL
Preparation of Cycloheximide Solution: Add
cycloheximide to 5.0mL of acetone. Mix thoroughly.
Bring volume to 100.0mL with distilled/deionized
water. Mix thoroughly. Filter sterilize.
Nystatin Solution:
Composition per 5.0mL:
Nystatin......................................................250,000U
Preparation of Nystatin Solution: Add nystatin
to distilled/deionized water and bring volume to
5.0mL. Mix thoroughly. Filter sterilize.
Polymyxin B Solution:
Composition per 2.0mL:
Polymyxin B ................................................10,000U
Preparation of Polymyxin B Solution: Add
polymyxin B to distilled/deionized water and bring
volume to 2.0mL. Mix thoroughly. Filter sterilize.
Nalidixic Acid Solution:
Composition per 2.0mL:
Nalidixic acid......................................................0.1g
NaOH (0.5N solution)..................................... 2.0mL
Preparation of Nalidixic Acid Solution: Add
nalidixic acid to 2.0mL of NaOH solution. Mix thor
oughly. Immediately before use, add 1.0mL of this
stock solution to 9.0mL of distilled/deionized water.
Mix thoroughly. Filter sterilize.
Vancomycin Solution:
Composition per 1.0mL:
Vancomycin ......................................................0.05g
Preparation of Vancomycin Solution: Add
vancomycin to distilled/deionized water and bring
volume to 1.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—ex
cept serum-glucose solution, bacitracin solution, cy
cloheximide solution, nystatin solution, polymyxin B
solution, nalidixic acid solution, and vancomycin so
lution—to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 10 psi pressure–
115°C. Cool to 50°C. Aseptically add 60.0mL of
sterile serum-glucose solution, 12.5mL of sterile ba
citracin solution, 10.0mL of sterile cycloheximide
solution, 2.0mL of sterile nystatin solution, 1.0mL of
sterile polymyxin B solution, 1.0mL of sterile nalid
ixic acid solution, and 0.4mL of sterile vancomycin
solution. Mix thoroughly. Pour into sterile Petri dish
es or distribute into sterile tubes. Allow tubes to cool
in a slanted position.
Use: For the selective isolation and cultivation of
Brucella species.