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SIM Motility Medium (BAM M137)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

SIM Motility Medium

(BAM M137)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Peptic digest of animal tissue..............................6.1g

Agar ....................................................................3.5g

Fe(NH4)2(SO4)2·6H2O ........................................0.2g

Na2S2O3·5H2O ....................................................0.2g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes in 15.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C. Allow tubes to

cool in an upright position.

Use: For the differentiation of members of the Entero

bacteriaceae based on H2S production, indole pro

duction, and motility.