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Simmons’ Citrate Agar (Citrate Agar) (BAM M138)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Simmons’ Citrate Agar

(Citrate Agar)

(BAM M138)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

NaCl....................................................................5.0g

Sodium citrate.....................................................2.0g

K2HPO4...............................................................1.0g

(NH4)H2PO4........................................................1.0g

MgSO4·7H2O......................................................0.2g

Bromthymol Blue .............................................0.08g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the differentiation of Gram-negative bacte

ria on the basis of citrate utilization. Bacteria that can

utilize citrate as sole carbon source turn the medium

blue.