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培养基配方
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Single-Layer Agar
[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]
Single-Layer Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1050.0mL:
Basal medium .................................................... 1.0L
Tributyrin substrate...........................................50.0g
pH 6.8 ± 0.2 at 25°C
Basal Medium:
Composition per liter:
Agar ..................................................................15.0g
Pancreatic digest of gelatin.................................5.0g
Beef extract.........................................................3.0g
Preparation of Basal Medium: Add agar to
1.0L of distilled/deionized water. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Tributyrin Substrate:
Composition:
Tributyrin substrate...........................................50.0g
Preparation of Tributyrin Substrate: Remove
free fatty acids in the tributyrin substrate by dissolv
ing 50.0g in 500.0mL of petroleum ether. Pass the so
lution through an activated alumina column. Remove
the petroleum ether by evaporation on a steam table
under 100% N2. Autoclave for 30 min at 15 psi pres
sure–121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine
1.0L of sterile basal medium with 50.0g of sterile
tributyrin substrate in a warm, sterile blender con
tainer. Blend for 1 min until homogenized. Rapidly
pour into sterile Petri dishes in 7.0mL volumes. Dry
the surface of the plates by partially opening the lids
in a laminar flow hood for 15 min.
Use: For the isolation, cultivation, and identification
of lipolytic microorganisms from food.