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Single-Layer Agar

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Single-Layer Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Basal medium .................................................... 1.0L

Fat substrate ......................................................50.0g

pH 6.8 ± 0.2 at 25°C

Basal Medium:

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Victoria Blue B solution ............................. 200.0mL

Preparation of Basal Medium: Add agar to

800.0mL of distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add 200.0mL of Victoria Blue B solu

tion. Mix thoroughly.

Victoria Blue B Solution:

Composition per 200.0mL:

Victoria Blue B .................................................0.12g

Preparation of Victoria Blue B Solution: Add

the Victoria Blue B to 200.0mL of distilled/deionized

water. Mix thoroughly. Filter sterilize. Warm to

50°C.

Fat Substrate:

Composition:

Fat substrate ......................................................50.0g

Preparation of Fat Substrate: Corn oil, soybean

oil, any cooking oil, lard, tallow, or triglycerides that

do not contain antioxidants or other inhibitory sub

stances may be used. Remove free fatty acids in the

fat substrate by dissolving 50.0g of fat substrate in

500.0mL of petroleum ether. Pass the solution

through an activated alumina column. Remove the

petroleum ether by evaporation on a steam table un

der 100% N2. Autoclave for 30 min at 15 psi pres

sure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine

1.0L of sterile basal medium with 50.0g of sterile fat

substrate in a warm, sterile blender container. Blend

for 1 min until homogenized. Rapidly pour into ster

ile Petri dishes in 7.0mL volumes. Dry the surface of

the plates by partially opening the lids in a laminar

flow hood for 15 min.

Use: For the isolation, cultivation, and identification

of lipolytic microorganisms from food.

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Single-Layer Agar