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Skim Milk Agar

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Skim Milk Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Agar ..................................................................15.0g

Pancretic digest of casein....................................5.0g

Yeast extract........................................................2.5g

Glucose ...............................................................1.0g

Skim milk solution...................................... 100.0mL

pH 7.0 ± 0.1 at 25°C

Preparation of Skim Milk Solution: Add skim

milk solids to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, ex

cept skim milk solution, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 100.0mL

of cooled, sterile skim milk solution. Mix thoroughly.

Pour into sterile Petri dishes or aseptically distribute

into sterile tubes.

Use: For the cultivation and differentiation of bacte

ria based on proteolytic activity.