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培养基配方
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Skirrow Brucella Medium
[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]
Skirrow Brucella Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Blood agar base No. 2................................. 940.0mL
Horse blood, lysed defibrinated.................... 50.0mL
Antibiotic solution ........................................ 10.0mL
pH 7.4 ± 0.2 at 25°C
Blood Agar Base No. 2
Composition per 940.0mL:
Proteose peptone...............................................15.0g
Agar ..................................................................12.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Liver digest .........................................................2.5g
pH 7.4 ± 0.2 at 25°C
Preparation of Blood Agar Base No. 2: Add
components to distilled/deionized water and bring
volume to 940.0mL. Mix thoroughly. Gently heat
while stirring and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Antibiotic Solution:
Composition per 10.0mL:
Vancomycin ......................................................0.01g
Trimethoprim...................................................5.0mg
Polymyxin B ...................................................2500U
Preparation of Antibiotic Solution: Add com
ponents to distilled/deionized water and bring vol
ume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 940.0mL of sterile
cooled blood agar base No. 2, aseptically add 50.0mL
of sterile, lysed defibrinated horse blood and 10.0mL
of sterile antibiotic solution. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of
Campylobacter species.