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SL Medium

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

SL Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

Glucose .............................................................10.0g

KH2PO4...............................................................6.0g

Arabinose............................................................5.0g

Sucrose................................................................5.0g

Yeast extract........................................................5.0g

Sodium acetate·3H2O..........................................2.5g

Diammonium citrate ...........................................2.0g

Tween 80.............................................................1.0g

MgSO4·7H2O ....................................................0.58g

MnSO4·4H2O ....................................................0.28g

pH 5.4 ± 0.2 at 25°C

Preparation of Medium: Add agar to 500.0mL

of distilled/deionized water. Gently heat and bring to

boiling. In a separate flask, add the remaining com

ponents, except sodium acetate, to distilled/deion

ized water and bring volume to 300.0mL. Mix

thoroughly. Combine the two solutions. In a separate

flask, add sodium acetate to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Ad

just pH to 5.4 with glacial acetic acid. Add this solu

tion to the agar solution. Mix thoroughly. Adjust pH

to 5.4 with additional glacial acetic acid. Bring vol

ume to 1.0L with distilled/deionized water. Do not

autoclave. Aseptically distribute into sterile screw

capped tubes or flasks.

Use: For the selective isolation and cultivation of

Lactobacillus species.